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人乳腺癌細(xì)胞(MCF7)

￥800.00元

細(xì)胞貨號(hào)： CL0206

細(xì)胞種屬： 人源細(xì)胞系

細(xì)胞別名： MCF7;MCF.7;MCF7;Michigan Cancer Foundation-7;ssMCF-7;ssMCF7;MCF7/WT;MCF7-CTRL;IBMF-7

發(fā)貨規(guī)格： 1×10?

供體信息： 69歲白人女性

腫瘤類(lèi)型： 乳腺癌

組織來(lái)源： 乳腺

背景信息

種屬：人源細(xì)胞系
組織來(lái)源：乳腺
腫瘤類(lèi)型：乳腺癌
供體信息： 69歲白人女性
背景描述： MCF7細(xì)胞是人類(lèi)乳腺癌研究中廣泛使用的研究模型，被廣泛用作激素依賴(lài)性乳腺癌的體外模型。MCF7細(xì)胞起源于一名患有轉(zhuǎn)移性腺癌的69歲白人女性的乳腺組織，是一種廣泛用于激素依賴(lài)性乳腺癌的體外模型，反映了LuminalA亞型。與更具侵襲性的乳腺癌相比，該亞型的特征是分級(jí)較低，預(yù)后較好。

培養(yǎng)建議

生長(zhǎng)形態(tài)：上皮細(xì)胞樣
生長(zhǎng)特性：貼壁
培養(yǎng)條件： DMEM/F12+5%馬血清+10ug/ml胰島素+20ng/ml表皮生長(zhǎng)因子+100ng/ml霍亂毒素+0.5ug/mL氫化可的松
凍存條件： 90%FBS+10%DMSO
血清使用等級(jí)：標(biāo)準(zhǔn)
傳代方式：胰酶消化
倍增時(shí)間： 24h
傳代比例：胰酶消化
換液頻率：每周2~3次
生物安全等級(jí)： 1
培養(yǎng)注意事項(xiàng)：

其他信息

蛋白表達(dá)： p53 negative,pGP9.5 negative,CEA positive
同工酶： PGM3,1,PGM1,1-2,ES-D,1-2,AK-1,1,GLO-1,1-2,G6PD,B,
基因表達(dá)： Thecellsexpressthewildtypeandvariantestrogenreceptorsaswellasprogesteronereceptor.Insulin-likegrowthfactorbindingproteins(IGFBP)BP-2,BP-4,BP-5
致癌基因： wnt7h+,Tx-4
致瘤性：裸鼠
突變信息： TP53 wt
核型： modal number = 82; range = 66 to 87.The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic.

人乳腺癌細(xì)胞

Catalog No CL0206

Product information

蛋白表達(dá)： p53 negative,pGP9.5 negative,CEA positive
同工酶： PGM3,1,PGM1,1-2,ES-D,1-2,AK-1,1,GLO-1,1-2,G6PD,B,
基因表達(dá)： Thecellsexpressthewildtypeandvariantestrogenreceptorsaswellasprogesteronereceptor.Insulin-likegrowthfactorbindingproteins(IGFBP)BP-2,BP-4,BP-5
致癌基因： wnt7h+,Tx-4
致瘤性：裸鼠
突變信息： TP53 wt
核型： modal number = 82; range = 66 to 87.The stemline chromosome numbers ranged from hypertriploidy to hypotetraploidy, with the 2S component occurring at 1%. There were 29 to 34 marker chromosomes per S metaphase; 24 to 28 markers occurred in at least 30% of cells, and generally one large submetacentric (M1) and 3 large subtelocentric (M2, M3, and M4) markers were recognizable in over 80% of metaphases. No DM were detected. Chromosome 20 was nullisomic and X was disomic.

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