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小鼠結(jié)締組織L細胞株929克隆(L929)

￥800.00元

細胞貨號： CL0339

細胞種屬： 小鼠細胞系

細胞別名： NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; L929; L929(NCTC); Clone 929; Earles's cells; Earle's L cells

發(fā)貨規(guī)格： 1×10?

供體信息： 100天雄性C3H/An小鼠

腫瘤類型：

組織來源： 正常皮下結(jié)締組織

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背景信息

種屬：小鼠細胞系
組織來源：正常皮下結(jié)締組織
腫瘤類型：
供體信息： 100天雄性C3H/An小鼠
背景描述： L-929 細胞是一種成纖維細胞樣細胞系，來源于 100 日齡雄性 C3H/An 小鼠的皮下結(jié)締組織。該細胞系成立于 1940 年代，由于其穩(wěn)健性、易于培養(yǎng)和應用多功能性，已成為各種生物和醫(yī)學研究領(lǐng)域的關(guān)鍵。L-929 細胞的特征在于其紡錘形、成纖維細胞形態(tài)和貼壁生長。它們廣泛用于細胞毒性測定，并作為評估材料生物相容性和各種物質(zhì)毒性作用的標準模型，這在生物材料和組織工程領(lǐng)域尤為重要。

培養(yǎng)建議

生長形態(tài)：成纖維細胞樣
生長特性：貼壁
培養(yǎng)條件： RPMI-1640+10%FBS+1%P/S
凍存條件： 90%FBS+10%DMSO
血清使用等級：標準
傳代方式：胰酶消化
倍增時間： 24~28h
傳代比例：胰酶消化
換液頻率：每周2~3次
生物安全等級： 1
培養(yǎng)注意事項：

其他信息

致瘤性： Yes, in immunosuppressed mice
抗原表達： H-2k
病毒： Ectromelia virus (mousepox): negative
病毒易感性： poliovirus 1, 2, 3, coxsackievirus B5, polyomavirus
逆轉(zhuǎn)錄酶：陽性
核型： modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

小鼠結(jié)締組織L細胞株929克隆

Catalog No CL0339

Product information

致瘤性： Yes, in immunosuppressed mice
抗原表達： H-2k
病毒： Ectromelia virus (mousepox): negative
病毒易感性： poliovirus 1, 2, 3, coxsackievirus B5, polyomavirus
逆轉(zhuǎn)錄酶：陽性
核型： modal chromosome number = 66; range = 65 to 68. There were approximately 20 to 30 marker chromosomes present in each metaphase spread. A high percentage of those markers were common to most analyzed cells. A long metacentric chromosome with secondary constriction was noted in 77/100 cells. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines.

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